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Nucleic Acids Research, 1987, Vol. 15, No. 10 4163-4178
© 1987

Articles

Lysozyme gene activity in chicken macrophages is controlled by positive and negative regulatory elements

Christof Steiner, Marc Muller, Aria Baniahmad and Rainer Renkawitz

Max-Planck-Institut für Biochemie Genzentrum, D-8033 Martinsried, FRG

Received March 10, 1987. Accepted April 27, 1987.

The chicken lysozyme gene is constitutively active in macrophages and under the control of steroid hormones in the oviduct.To investigate which DNA elements are involved In the control of its expression in macrophages we performed transient DNA transfer experiments with two different types of plasmids: 5'-deletion mutants of the upstream region of the chicken lysozyme gene and different fragments from this area in front of the thymidine kinase promoter (herpes simplex virus), each placed in front of the CAT (chloramphenlcol acetyl transferase) coding sequence. Two enhancers (E-2.7kb and E-0.2kb) were characterized. They are active in macrophages, but not in chicken flbroblasts. Furthermore a negative element (N-2.4kb) was Identified, which is active in fibroblasts and promyelocytes, but not in mature macrophages. The combined action of all three elements contributes to the observed lysozyme gene activities: no activity In fibroblasts, moderate activity in promyelocytes and high activity in mature macrophages.


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