Skip Navigation

This Article
Right arrow Print PDF (593K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (84)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Shibahara, S.
Right arrow Articles by Morisawa, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shibahara, S.
Right arrow Articles by Morisawa, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 11 4403-4415
© 1987

Articles

Site-directed cleavage of RNA

Susumu Shibahara, Faculty of Pharmaceutical Sciences, Sachiko Mukai, Tohru Nishihara1, Hideo Inoue2, Eiko Ohtsuka, Faculty of Pharmaceutical Sciences2 and Hirokazu Morisawa

1Department of Biochemistry, Kawasaki Medical School Kurashiki 701–01 2Hokkaido University Sapporo 060, Japan Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, Kawasaki 210

Received March 18, 1987. Accepted April 30, 1987.

Using complementary chimeric oligonucleotides containing deoxyribonucleotides and 2'-O-methylribonucleotides (1), enzymatically synthesized RNA (90 mer) were cleaved at a single site with Eschericia coli RNaseH, either at a hairpin loop or at a stem region. Especially, site-specific cleavage occured in even the target region being enclosed within a stable, base-paired stem. The method is proved to be generally applicable to RNA containing secondary structures.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
Q. M. Mitrovich and P. Anderson
Unproductively spliced ribosomal protein mRNAs are natural targets of mRNA surveillance in C. elegans
Genes & Dev., September 1, 2000; 14(17): 2173 - 2184.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
T. P. Rasmussen and M. R. Culbertson
The Putative Nucleic Acid Helicase Sen1p Is Required for Formation and Stability of Termini and for Maximal Rates of Synthesis and Levels of Accumulation of Small Nucleolar RNAs in Saccharomyces cerevisiae
Mol. Cell. Biol., December 1, 1998; 18(12): 6885 - 6896.
[Abstract] [Full Text]

Home page
 ScienceHome page
P. Schultz
The interplay between chemistry and biology in the design of enzymatic catalysts
Science, April 22, 1988; 240(4851): 426 - 433.
[Abstract] [PDF]

Home page
 ScienceHome page
D. Corey and P. Schultz
Generation of a hybrid sequence-specific single-stranded deoxyribonuclease
Science, December 4, 1987; 238(4832): 1401 - 1403.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.