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Nucleic Acids Research, 1987, Vol. 15, No. 11 4417-4436
© 1987

Articles

RNA polymerase II terminates transcription in vitro in the SV40 origin region

David S. Grass, Richard Jove and James L. Manley

Department of Biological Sciences, Columbia University New York, NY 10027, USA

Received March 6, 1987. Revised May 12, 1987. Accepted May 12, 1987.

To begin to study signals on DNA that can cause mammalian RNA polymerase II to terminate transcription, we examined the RNA transcripts produced from a number of different DNA templates in a HeLa whole-cell extract. When transcripts initiating from the strong adenovirus late promoter and extending through the SV40 promoter-replication origin region were analyzed, it was observed that a significant fraction (–75%) were terminated within these SV40 sequences. These transcripts, the 3' ends of which were mapped to several sites within an approximately 200 base pair region, appeared to result from transcription termination, as judged by kinetic and pulse-chase experiments. The possible significance of these findings with respect to SV40 gene expression in particular and transcription termination in general are discussed.


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