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Nucleic Acids Research, 1987, Vol. 15, No. 11 4437-4448
© 1987

Articles

Cosmid mapping of the human chorionic gonadotropin ß subunit genes by field-inversion gel elect rophoresis

Madge Y. Graham, Tetsuo Otani, Irving Boime, Maynard V. Olson1, Georges F. Carle1 and David D. Chaplin2

1Departments of Genetics, Washington University School of Medicine St Louis, MO 63110, USA 2Departments of Medicine, the Howard Hughes Medical Institute, Washington University School of Medicine St Louis, MO 63110, USA Departments of Pharmacology, Washington University School of Medicine St Louis, MO 63110, USA

Received February 27, 1987. Revised May 12, 1987. Accepted May 12, 1987.

A cosmid clone containing the entire hCGß gene cluster has been isolated. The restriction map of this clone has been determined by an indirect-end-label FIGE (field inversion gel electrophoresis) method. Analysis of this cosmid clone shows that there are 6 hCGß genes in human genomic DNA. A previously uncloned portion of the hCGß cluster, termed the "gap" region, has been shovn not to contain any sequences homologous to the hCGß cDNA. The restriction mapping method employed in this study takes advantage of the superior resolution of FIGE for high molecular weight DNA fragments in the size range 15–50 kb. This method is broadly applicable and permits rapid and accurate restriction mapping for extended regions of genomic DNA that have been cloned into cosmid or lambda vectors.


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