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Nucleic Acids Research, 1987, Vol. 15, No. 11 4481-4489
© 1987

Articles

An electrophoretic karyotype for Schizosaccharomyces pombe by pulsed field gel electrophoresis

Cassandra L. Smith, Tomohiro Matsumoto, Osami Niwa, Stephanie Klco, Jian-Bin Fan, Mitsuhiro Yanagida and Charles R. Cantor

Department of Genetics and Development, College of Physicians and Surgeons, Columbia University New York, NY 10032, USA Department of Biophysics, Faculty of Sciences, Kyoto University Sakyo-ku, Kyoto 606, Japan

Received February 12, 1987. Revised May 5, 1987. Accepted May 5, 1987.

The three chromosomal DNAs of S. pombe have been fractionated by pulsed field gel electrophoresis. The resulting molecular karyotype will greatly speed gene mapping in this organism, and it indicates that the separation range of the technique extends to DNA molecules as large as 9,000,000 base pairs.


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