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Nucleic Acids Research, 1987, Vol. 15, No. 12 4889-4899
© 1987

Articles

c-myc protein can be substituted for SV40 T antigen in SV40 DNA replication

Sanae M.M. Iguchi-Ariga, Teru Itani, Masamitsu Yamaguchi1 and Hiroyoshi Ariga*

1Aichi Cancer Center, Research Institute Chikusa-ku, Nagoya 464, Japan The Institute of Medical Science, The University of Tokyo 4-6-1 Shirokanedai, Minato-ku, Tokyo 108

*To whom correspondence should be addressed

Received April 13, 1987. Revised May 18, 1987. Accepted May 18, 1987.

Replicating activity of SV40 origin-containing plasmid was tested in human cells as well as in monkey CosI cells. All the plasmids possessing SV40 ori sequences could replicate, even in the absence of SV40 T antigen, in human HL-60 and Raji cells which are expressing c-myc gene at high level. The copy numbers of the replicated plasmids in these human cells were 1/100 as high as in monkey CosI cells which express SV40 T antigen constitutively. Exactly the same plasmids as the transfected original ones were recovered from the Hirt supernatant of the transfected HL-60 cells. Furthermore, replication of the SV40 ori-containing plasmids in HL-60 cells was inhibited by anti-c-myc antibody co-transfected into the cells. These results indicate that the c-mic protein can be substituted for SV40 T antigen in SV40 DNA replication.


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