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Nucleic Acids Research, 1987, Vol. 15, No. 15 6243-6259
© 1987

Articles

A transcriptional activator is located in the coding region of the yeast PGK gene

J. Mellor, M. J. Dobson1, A. J. Kingsman and S. M. Kingsman

Department of Biochemistry, University of Oxford South Parks Road, Oxford OX1 3QU 1Department of Botany, School of Biological Sciences, University of Nottingham University Park, Nottingham N67 2RD, UK

Received May 5, 1987. Accepted July 2, 1987.

Expression of heterologous genes from the PGK promoter on high copy number plasmids in yeast is relatively poor compared to the intact PGK gene because of low steady-state RNA levels. In this paper we show that low levels of heterologous RNA are not due to instability of mRNA but result from inefficient transcription due to a defect in RNA synthesis. A comparison of RNA levels from homologous and heterologous transcription units allowed the identification of a positive activator for transcription within the PGK coding region which is required for efficient expression of the PGK gene. Deletion of this region, the "downstream activator sequence", causes a six to ten fold reduction in transcriptional efficiency from the PGK 5' non-coding region.


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