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Nucleic Acids Research, 1987, Vol. 15, No. 16 6315-6329
© 1987

Articles

Site specificity of bleomycin cleavage in purified and intracellular simian virus 40 DNA

Julia E. Griinwade+, Emily B. Cason and Terry A. Beerman*

Grace Cancer Drug Center, Roswell Park Memorial Institute 666 Elm Street, Buffalo, NY 14263, USA

*To whom reprint requests should be addressed

Received June 4, 1987. Revised July 20, 1987. Accepted July 20, 1987.

The sites of bleomycin-induced cleavage of purified and intracellular simian virus 40 DNA were examined. Breaks 1n purified DNA were mapped to several discrete sites that were distributed throughout the viral genome, but were not associated with a common genetic element. Double-stranded breaks were made in positions of the first single-stranded nick, and regions of cuts were unaffected by changes in DNA superhelicity. Bleomydn cut intracellular chromosomes at the same sites that were cleaved fy purified DNA. These results indicate that SV40 DNA contains DNA secondary structures that are highly preferred sites for BLM cleavage. These conformations appear to be unaffected by nucleoproteins bound to DNA.


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