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Nucleic Acids Research, 1987, Vol. 15, No. 16 6349-6367
© 1987

Articles

Generation of linear multigenome-length plasmid molecules in Bacillus subtilis

Jean-Francois Viret and Juan C. Alonso*

Max-Planck-Institut für Molekulare Genetik Ihnestrasse 73, D-1000 Berlin 33, FRG

*To whom correspondence should be addressed

Received June 1, 1987. Revised July 7, 1987. Accepted July 7, 1987.

Linear multigenome-length double and single stranded plasmid DNA was identified in a Bacillus subtilis ATP-dependent DNAase mutant strain (addA5) bearing plasmids pC194 or pBD95ts. Plasmid pBC30, a seg mutant of pC194, as well as some pUB110 derivatives with rearrangements external to the minimal replicon, produce high amounts of such a concatemeric DNA, even in Rec + cells. The synthesis of this type of plasmid DNA occurs in the absence of an active plasmid-encoded Rep protein and is markedly affected in polA5 and recE4 genetic backgrounds. To account for these observations, we propose that the AddAB complex serves to prevent a sigma-type replication of plasmid DNA.


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