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Nucleic Acids Research, 1987, Vol. 15, No. 16 6387-6403
© 1987

Articles

Analysis of transcripts of the major cluster of tRNA genes in the mitochondrial genome of S. cerevisiae

S. Francisci, C. Palleschi, A. Ragnini and L. Frontali

Department of Cell and Developmental Biology, University of Rome I Rome, Italy

Received May 19, 1987. Revised July 31, 1987. Accepted July 31, 1987.

The transcripts of a 6Kbp region of the mitochondrial genome of S.cerevisiae, localized in the 21S rRNA-OXIl span and including 12 tRNA genes (from tRNAthr to tRNAala)and several G+C clusters, have been studied by analysis of in vitro capped primary transcripts and by fine mapping of the 5' ends of transcripts.

The study was performed in the w.t. strain D273-1OB and in several rho mutants retaining different, partially overlapping portions of the studied region; the mutants accumulate incompletely-processed precursors of tRNAs due to the absence of the tRNA synthesis locus.

Results show the presence in the region of four sites at which initiation occurs at a consensus nonanucleotide TTATAAGTA (or a minor variant of the same); however different initiation sites are used in different strains, and several differences as compared to initiation in vitro can also be observed. Termini arising by processing are often localized at AATATAA or AATATATTTT sequences localized immediately adjacent to a G+C cluster or a tRNA sequence.


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