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Nucleic Acids Research, 1987, Vol. 15, No. 16 6489-6500
© 1987

Articles

Identification of nuclear cap specific proteins in HeLa cells

Florence Rozen1 and Nahum Sonenberg1,2,*

1Department of Biochemistry, McGill University Montréal, Quebec H3G 1Y6, Canada 2McGill Cancer Center, McGill University Montréal, Quebec H3G 1Y6, Canada

*To whom correspondence should be addressed at: Department of Biochemistry, McGill University, 3655 Drummond Street, Montréal, Quebec H3G 1Y6, Canada

Received May 1, 1987. Revised July 23, 1987. Accepted July 23, 1987.

Two polypeptides of apparent molecular mass of 20 and 115 kilodaltons in nuclear fractions from HeLa cells were shown to recognize and be crosslinked to the cap structure of eukaryotic mRNAs in a cap-dependent fashion. Crosslinking of the 20 and 115 kDa polypeptides was sensitive to inhibition by low concentrations of the cap analogue m7GDP and resistant to inhibition by high KC1 concentrations. In addition, crosslinking of these polypeptides to the cap structure occurred in nuclear extracts prepared from poliovirus-infected cells, under conditions where cytoplasmic cap binding proteins were incapable of interacting with the mRNA cap structure. The possible function of nuclear cap binding proteins is discussed.


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