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Nucleic Acids Research, 1987, Vol. 15, No. 16 6665-6676
© 1987

Articles

The effect of nucleotide substitution on DNA denaturation profiles

I.V. Razlutskii, L.S. Shlyakhtenko and Yu. L. Lyubchenko*

Institute of Molecular Genetics, USSR Academy of Sciences Kurchatov Sq. 46, Moscow 123182, USSR

*To whom correspondence should be addressed

Received April 10, 1987. Revised July 20, 1987. Accepted July 20, 1987.

The melting profiles were obtained for DNA restriction fragments of approx. 1150 bp with deletion of one, five or six base pairs making them different from each other. In all cases the deletions caused a shift of one melting peak without affecting the positions of the other three peaks. The effect amounted to 0.28+ 0.03 C upon the deletion of one GC pair. The melting of DNS fragments was also studied by electrophoresis in denaturing gradient gels. The deletion of one GC pair was shown to cause an appreciable shift of the electrophoretic denaturation profile.


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