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Nucleic Acids Research, 1987, Vol. 15, No. 16 6691-6704
© 1987

Articles

The right end of transposable bacteriophage D108 contains a 520 base pair protein-encoding sequence not present in bacteriophage Mu

George B. Szatmari1, Martine Lapointe2 and Michael S. DuBow*

Department of Microbiology and Immunology, McGill University 3775 University Street, Montreal, Quebec H3A 2B4, Canada

*To whom correspondence should be addressed

Received March 26, 1987. Revised July 16, 1987. Accepted July 16, 1987.

We have cloned and characterized the right end terminal 796 bp of the transposable Mu-like bacteriophage D108. This region encompasses a 520 bp region of D108-specific sequences not present in phage Mu that contain an open reading frame encoding a 12 KDa protein. This protein can be visualized in vivo when the region is placed downstream from the strong lac UV5 promoter. The open reading frame can be expressed from the dam-regulatedmod promoter (for modification of D108 DNA), yet also contains its own dam-independent promoter for expression that is detectable by northern blot analysis late in the D106 lytic cycle. Comparison of this region of D108 DNA with the corresponding region of Mu DNA suggests that a complex rearrangement has occurred at the phages' right ends during their evolution.

1Present addresses: Institute of Genetics, University of Glasgow, Church Street, Glasgow Gil 5J6, UK

2Department of Endocrinology, Montreal Children's Hospital, 2300 Tupper Street, Montreal, Quebec H3G 1A4, Canada


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