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Nucleic Acids Research, 1987, Vol. 15, No. 17 6799-6811
© 1987

Articles

Structure of tobacco genes encoding pathogenesis-related proteins from the PR-1 group

Ben J.C. Cornelissen+, Jeannine Horowitz1, Jan A.L. van Kan, Robert B. Goldberg1 and John F. Bol*

Department of Biochemistry, Leiden University Wassenaarseweg 64, 2333 AL Leiden, The Netherlands 1Department of Biology, University of California Los Angeles, CA 90024, USA

*To whom correspondence should be addressed

Received July 17, 1987. Accepted August 15, 1987.

Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) was found to induce the synthesis of mRNA encoding a basic protein with a 67 % amino acid sequence homology to the known acidic pathogenesis-related (PR) proteins la, lb and lc. By Southern blot hybridization it was shown that the tobacco genome contains at least eight genes for acidic PR-1 proteins and a similar number of genes encoding the basic homologues. Clones corresponding to three of the genes for acidic PR-1 proteins were isolated from a genomic library of Samsun NN tobacco. The nucleotide sequence of these genes and their flanking sequences were determined. One clone was found to correspond to the PR-1a gene; the two other clones do not correspond to known TMV-1nduced PR-1 mRNA's and may represent silent genes. Compared to the PR-1a gene, these genes contain an Insertion or deletion in the putative promoter region and mutations affecting the PR-1 reading frame.

+Present address: MOGEN International B.V., Einsteinweg 97, 2333 CB Leiden, The Netherlands


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