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Nucleic Acids Research, 1987, Vol. 15, No. 17 6899-6916
© 1987

Articles

Human DNA (cytosme-5)methyftransferase selectively methylates duplex DNA containing mispairs

Steven S. Smith*, Thomas A. Hardy and David J. Baker

Division of Surgery, City of Hope National Medical Center 1500 E.Duarte Rd, Duarte, CA 91010, USA

*To whom correspondence should be addressed

Received May 8, 1987. Revised August 6, 1987. Accepted August 6, 1987.

The presence of the C.C mispair in a defined duplex oligodeoxy-nucleotide enhanced its capacity to serve as a substrate for highly purified human DNA methyltransferase. Analysis of tritiated reaction products showed that the C.C mispair acted as a "methylation acceptor" in that it was itself rapidly methylated. The m5 C.G base pair also enhanced the capacity of the oligodeoxynucleotide to serve as a substrate for the enzyme. However, this complementary base pair was found to act as a "methylation director". That is, the presence of the m5C in one strand induced the enzyme to rapidly methylate at the cytosine residue on the opposite strand in an adjacent C.G base pair.


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