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Nucleic Acids Research, 1987, Vol. 15, No. 17 7081-7090
© 1987

Articles

Characterization of the small RNA of the bacteriophage ø29 DNA packaging machine

Peixuan Guo*, Suzanne Bailey1, James W. Bodley1 and Dwight Anderson

Department of Microbiology/Dentistry, University of Minnesota Minneapolis, MN 55455, USA 1Department of Biochemistry, University of Minnesota Minneapolis, MN 55455, USA

Received June 5, 1987. Accepted July 29, 1987.

The prohead connector of the bacteriophage ø29 DNA packaging machine was reconstructed with the small RNA that regulates DNA packaging in vitro. The complete sequence of the 120 nucleotide RNA proved its origination from the promoter PE1(A1) of the left early region of ø29 DNA, the end packaged first during assembly. The prohead RNA was clearly distinct from eubacterial 5S rRNA in sequence and composition.

*Permanent address: South China Agricultural University, Guanzhou (Canton), The People's Republic of China


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