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Nucleic Acids Research, 1987, Vol. 15, No. 18 7357-7368
© 1987

Articles

Transcription of the intergenic regions of the tubulin gene cluster of Trypanosoma brucei: evidence for a polyclstronic transcription unit in a eukaryote

Martin A. Imboden+, Peter W. Laird1, Marianne Affolter and Thomas Seebeck*

Institute of General Microbiology Baltzerstrasse 4, 3012 Bern, Switzerland 1The Netherlands Cancer Institute Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands

+To whom correspondence should be addressed

Received July 6, 1987. Revised August 27, 1987. Accepted August 27, 1987.

The tubulin genes of T- brucei are clustered 1n a tightly packed array of alternating {alpha}- and ß-genes. The steady state mRNA contains one abundant mRNA species each for {alpha}- and ß-tubulin, both carrying the identical 35 nt mini-exon sequence at their 5'-ends.

We have used in vitro run-on transcription assays to investigate the mechanism of tubulin gene transcription in T. brucei. Our results show that the regions between the individual tubulin genes are transcribed at the same rate as are the genes themselves. On the other hand, transcripts containing the intergenic regions could not be detected by Northern analysis or in vivo labelling experiments. We conclude that putative transcripts from the intergenic regions have a half-life of less than one minute. These results suggest that the tubulin gene cluster is transcribed as a single contiguous transcription unit yielding a primary transcript which is rapidly processed into individual mRNAs by the polyadenylation and mini-exon trans splicing machineries.

*Present address: Department for Developmental Biology, Baltzerstrasse 4, CH-3012 Bern, Switzerland


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