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Nucleic Acids Research, 1987, Vol. 15, No. 18 7463-7476
© 1987

Articles

Structural and functional characterization of a Dictyostetiumgene encoding a DIF inducible, prestalk-enriched mRNA sequence

A. Ceccarelli*, S.J. McRobbie, K.A. Jermyu, K. Duffy, A. Early and J.G. Williams

Imperial Cancer Research Fund, Clare Hall Laboratories Blanche Lane, South Minims, Potters Bar, Hertfordshire EN6 3LD, UK

The pDd56 mRNA sequence is highly enriched in prestalk over prespore cells and is inducible by DIF, the putative Dictyostelium stalk-specific morphogen. We show that the pDd56 gene is composed of forty one copies of a twenty four amino acid, cysteine rich repeat. This is highly homologus to a repeat which we have previously shown to compose the major fraction of the pDd63 mRNA, another DIF inducible, prestalk-enriched sequence. The predicted pDd56 protein contains a putative signal peptide but does not appear to contain a transmembrane segment. In combination these features suggest it to be an extrinsic protein and we confirm this elsewhere by showing that the pDd56 gene encodes a known, extracellular protein of the stalk. The pDd56 mRNA is dependent upon exogenous DIF for its accumulation. We show that this control is exerted at the transcriptional level and that a restriction fragment containing 1.7Kb of upstream sequence directs temporally-regulated expression of the gene.

*Present address: The Hygiene Institute, Cattedra di Biologie Generate. Via Santena 5 bis. Tonno 10126, Italy


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