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Nucleic Acids Research, 1987, Vol. 15, No. 18 7531-7547
© 1987

Articles

2D-NMR studies of the unnatural duplex {alpha}-d(TCTAAAC)-ß-d(AGATTTG)

G. Lancelot, J.-L. Guesnet, V. Roig and N.T. Thuong

Centre de Biophysique Moléculaire CNRS, 45071 Orléans Cedex 2, France

Received June 29, 1987. Revised August 10, 1987. Accepted August 10, 1987.

The unnatural oligonucleotide {alpha}-d(TCTAAAC) was synthesized and was found more resistant towards endonucleases than its ß-analog. 2D-NMR experiments allowed the assignment of all non-exchangeable aromatic and sugar protons except for the overlapping 5''-5'' resonances, as well as the exchangeable imino protons of the parallel hybrid duplex {alpha}-d(TCTAAAC) - ß-d(AGATTTG). NMR studies show that the strength of the association between the {alpha}-strand and the ß parallel strand is equivalent to that between their anti-parallel complementary ß-analogs ß-d(CAAATCT) and ß-d(AGATTTG). NOE data provide evidence that both duplexes form stable right-helical duplexes with an anti-conformation on the glycosyl linkages and a Watson-Crick pairing. NOESY and COSY spectra allowed us to determine that {alpha} and ß deoxyriboses adopt a 3'-exo conformation.


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