Skip Navigation

This Article
Right arrow Print PDF (467K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Purvis, I. J.
Right arrow Articles by Brown, A. J.P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Purvis, I. J.
Right arrow Articles by Brown, A. J.P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 19 7963-7974
© 1987

Articles

Translation and stability of an Escherichia coli ß-galactosidase mRNA expressed under the control of pyruvate kinase sequences in Saccharomyces cerevisiae

Ian J. Purvis, Lynn Loughlin, Andrew J.E. Bettany and Alistair J.P. Brown*

Institute of Genetics, University of Glasgow Church Street, Glasgow G11 5JS, UK

*To whom correspondence should be addressed

Received July 31, 1987. Accepted September 10, 1987.

Plasmids were assembled in which the coding region of the pyruvate kinase (PYK) gene of Saccharomvces cerevisiae was replaced by that of the B-galactosidase (LacZ) gene from Escherichia coli. Analysis of the resultant, chimaeric transcripts from low copy number, centromeric plasmids indicated that this substitution caused a dramatic reduction in the steady-state level of the messenger RNA (mRNA). This fluctuation cannot be wholly accounted for by the 2-fold decrease in mRNA stability observed. This is consistent with the existence of a transcriptional Downstream Activation Site (DAS) within the PYK coding region, analogous to the DAS reported within the yeast phosphoglycerate kinase gene (PGK; Kingsman, S M et al. (1985) Biotech. Gen, Eng. Rev. 3. 377). At these low levels of heterologous gene expression, comparison of the distribution of PYK and PYK/LacZ transcripts across polysome gradients revealed no significant effect mediated by their striking disparity in codon usage. Nevertheless, upon increasing B-galactosidase mRNA levels, via manipulation of plasmid copy number, a distinct decline in ribosome loading was observed for the heterologous PYK/LacZ transcript which was not mirrored by either endogenous PYK transcripts or other yeast mRNAs of high (Ribosomal protein 1) or moderate (Actin) codon bias. However, high levels of the PYK/LacZ mRNA did affect the translation of an endogenous mRNA with poor codon bias (TRP2). The possible basis for this phenomenon is discussed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
P. Wenz, S. Schwank, U. Hoja, and H.-J. Schuller
A downstream regulatory element located within the coding sequence mediates autoregulated expression of the yeast fatty acid synthase gene FAS2 by the FAS1 gene product
Nucleic Acids Res., November 15, 2001; 29(22): 4625 - 4632.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
W. Tao, R. J. Deschenes, and J. S. Fassler
Intracellular Glycerol Levels Modulate the Activity of Sln1p, a Saccharomyces cerevisiae Two-component Regulator
J. Biol. Chem., January 1, 1999; 274(1): 360 - 367.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. A. Pedersen, J. H. Rasmussen, and P. L. Jørgensen
Expression in High Yield of Pig alpha1beta1 Na,K-ATPase and Inactive Mutants D369N and D807N in Saccharomyces cerevisiae
J. Biol. Chem., February 2, 1996; 271(5): 2514 - 2522.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.