Skip Navigation

This Article
Right arrow Print PDF (3422K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (49)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Loidl, P.
Right arrow Articles by Gröbner, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Loidl, P.
Right arrow Articles by Gröbner, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 20 8351-8366
© 1987

Articles

Postsynthetic acetylation of histones during the cell cycle: a general function for the displacement of histones during chromatin rearrangements

Peter Loidl* and Peter Gröbner

Institut für Medizinische Chemie und Biochemie der Universitaät Fritz-Preglstr.3, 6020 Innsbruck, Austria

*To whom correspondence should be addressed

Received June 26, 1987. Revised September 14, 1987. Accepted September 14, 1987.

Postsynthetic acetylation of core histones exhibits a peak during S-phase of the Physarum cell cycle. The maximum 3H-acetate incorporation precedes the maximum of hi stone synthesis. Acetate is incorporated into all core histones during S-phase, but only into H2A and H2B during G2-per1od. Resolution of acetylated H4-subspecies reveals acetate incorporation into preexisting H4, but not into newly synthesized molecules during mitosis and early S-phase. In a prota-m1ne competition assay histones from S-phase chromatin are released at lower protamine concentrations as compared to the lower acetylated G2-chromation. We demonstrate a preferential release of highly acetylated H4-subspecies at low protamine concentrations. Our results fit into a general model of the relationship between histone acetylation and chromatin assembly. According to this model acetylation of core histones would serve as a signal for displacement of histones from nucleosomes by modulating hi stone-protein or h1stone-DNA Interactions. We propose that this mechanism operates during DNA-replication and transcription, as well as during other chromatin rearrangements.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
A.K. Faure, C. Pivot-Pajot, A. Kerjean, M. Hazzouri, R. Pelletier, M. Peoc'h, B. Sele, S. Khochbin, and S. Rousseaux
Misregulation of histone acetylation in Sertoli cell-only syndrome and testicular cancer
Mol. Hum. Reprod., December 1, 2003; 9(12): 757 - 763.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. Jaboin, J. Wild, H. Hamidi, C. Khanna, C. J. Kim, R. Robey, S. E. Bates, and C. J. Thiele
MS-27-275, an Inhibitor of Histone Deacetylase, Has Marked in Vitro and in Vivo Antitumor Activity against Pediatric Solid Tumors
Cancer Res., November 1, 2002; 62(21): 6108 - 6115.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
S Lang, T Decristoforo, W Waitz, and P Loidl
Biochemical and morphological characterization of the nuclear matrix during the synchronous cell cycle of Physarum polycephalum
J. Cell Sci., January 8, 1993; 105(4): 1121 - 1130.
[Abstract] [PDF]

Home page
 ScienceHome page
P. Megee, B. Morgan, B. Mittman, and M. Smith
Genetic analysis of histone H4: essential role of lysines subject to reversible acetylation
Science, February 16, 1990; 247(4944): 841 - 845.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.