Nucleic Acids Research, 1987, Vol. 15, No. 20 8367-8385
© 1987
Articles |
Upstream regulatory regions required to stabilize binding to the TATA sequence in an adesovirus early promoter
Division of Hematology-Oncology 11-242 Louis Factor Building Department of Medicine, UCLA School of Medicine and Jonnson Comprehensive Cancer Center Los Angeles, CA 90024, USA
Received June 24, 1987. Revised September 1, 1987. Accepted September 1, 1987.
Of the five early adenovirus promoters, the early region 3 (E3) promoter is one of the most strongly induced by the E1A protein. To identify cellular proteins involved in both the basa1 and ElA-induced transcriptional regulation of the E3 promoter, ONase I footprinting using partially purified Hela cell extracts was performed. Four regions of the E3 promoter serve as binding domains for cellular proteins. These regions are found between –156 to –179 (site IV), –83 to –103 (site III), –47 to –67 (site II), and –16 to –37 (site I), relative to the start of transcription. Examination of the DNA sequences in each binding domain suggests that site III likely serves as a binding site for activator protein 1 (AP-1), site II for the cyclic AMP regulatory element binding protein (CREB), and site I for a TATA binding factor. The factors binding to either site II or III were sufficient to stabilize binding to the TATA sequence (site I). Mutagenesis studies Indicated that both sites II and III, in addition to site I, are needed for complete basal and ElA-induced transcription. These results suggest that multiple cellular factors are Involved in both the basal and EIA-induced transcriptional regulation of the E3 promoter, and that either of two upstream regions are capable of stabilizing factor binding to the TATA sequence.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Ye and D. J. Pintel Upstream AP1- and CREB-Binding Sites Confer High Basal Activity on the Adeno-Associated Virus Type 5 Capsid Gene Promoter J. Virol., March 15, 2007; 81(6): 2605 - 2613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Stearns, G. Kim, F. Garcia, and M. Wang Interleukin-10 Induced Activating Transcription Factor 3 Transcriptional Suppression of Matrix Metalloproteinase-2 Gene Expression in Human Prostate CPTX-1532 Cells Mol. Cancer Res., July 1, 2004; 2(7): 403 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Mahr, J. M. Boss, and L. R. Gooding The Adenovirus E3 Promoter Is Sensitive to Activation Signals in Human T Cells J. Virol., December 20, 2002; 77(2): 1112 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Hansen, D. Holm, V. Moeller, L. Vitved, C. Bendixen, K. B. M. Reid, K. Skjoedt, and U. Holmskov CL-46, a Novel Collectin Highly Expressed in Bovine Thymus and Liver J. Immunol., November 15, 2002; 169(10): 5726 - 5734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Deryckere and H.-G. Burgert Tumor Necrosis Factor alpha Induces the Adenovirus Early 3Promoter by Activation of NF-kappa B J. Biol. Chem., November 22, 1996; 271(47): 30249 - 30255. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D A Engel, S Hardy, and T Shenk cAMP acts in synergy with E1A protein to activate transcription of the adenovirus early genes E4 and E1A. Genes & Dev., December 1, 1988; 2(12a): 1517 - 1528. [Abstract] [PDF]
|
|
|
|
|
|
N C Jones, P W Rigby, and E B Ziff Trans-acting protein factors and the regulation of eukaryotic transcription: lessons from studies on DNA tumor viruses. Genes & Dev., March 1, 1988; 2(3): 267 - 281. [PDF]
|
|