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Nucleic Acids Research, 1987, Vol. 15, No. 20 8521-8530
© 1987

Articles

Competition between sigma factors for core RNA polymerase

Sohail Malik, Katya Zalenskaya and Alexander Goldfarb

Department of Microbiology, Columbia University College of Physicians and Surgeons 701 West 168 Street, New York, NY 10032, USA

Received June 24, 1987. Revised September 22, 1987. Accepted September 22, 1987.

The switch of RNA polymerase specificity from early to late promoters of bacteriophage T4 is achieved by substitution of host sigma factor, {sigma}, with the T4 induced factor, {sigma}gp55. However, overproduction of {sigma}gp55 from an expression vector is not detrimental to Escherichia coli growth. Direct competition binding assays demonstrate that {sigma}70 readily displaces {sigma}gp55 from RNA polymerase and thereby reverses the promoter specificity of the enzyme. The displacement also occurs with the core enzyme modified by bacteriophage T4 infection. We postulate that an antagonist of {sigma}70 should be formed in T4-infected cells to aid {sigma}gp55{sigma}gp55 in the early/late switch.


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