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Nucleic Acids Research, 1987, Vol. 15, No. 21 8607-8620
© 1987

Articles

Messenger RNAs from the E1 region of bovine papillomavirus type 1 detected in virus-infected bovine cells

Stanley Burnett, Jorge Moreno-Lopez1 and Ulf Pattersson

Department of Medical Genetics, Biomedical Center Box 589, University of Uppsala, S-751 23 Uppsala 1Department of Veterinary Microbiology (Virology), Swedish University of Agricultural Sciences S-751 23 Uppsala, Sweden

Received August 14, 1987. Revised October 2, 1987. Accepted October 2, 1987.

Bovine papillomavirus type 1 DNA replicated to a high copy number in virus-infected bovine fibroblasts. infected bovine cells were therefore used as a source of RNA for Northern blotting analysis to search for viral transcripts hybridizing to the E1 gene region, implicated in viral DNA repliction. Cytoplasmic polyadenylated RNA preparations contained at least five different E1-region transcripts, ranging from 1200 to approximately 4500 nucleotides in length. All of these species contained sequence information from the 5'-end of the E1 open reading frame, but only the largest species included sequences from its central portion. The latter transcript is a candidate mRNA for a stimulatory replication factor (R) previously mapped by genetic experiments (1).


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