Skip Navigation

This Article
Right arrow Print PDF (5351K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Barrett, P.
Right arrow Articles by Sommerville, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barrett, P.
Right arrow Articles by Sommerville, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 21 8679-8691
© 1987

Articles

An alternative protein factor which binds the internal promoter of Xenopus 5S ribosomal RNA genes

Perry Barrett and John Sommerville

Department of Biology, University of St Andrews St Andrews, Fife KY16 9TS, UK

Received August 5, 1987. Revised October 8, 1987. Accepted October 8, 1987.

In small oocytes of Xenopus species, two sets of 5S RNA genes, oocytetype and somatic-type, are fully activated. The 5S RNA transcripts are temporarily stored, half in association with TFIIIA to form a 7S particle, the other half in association with tRNA and two proteins (p48 and p43) to form a 42S particle. It has been established previously that TFIIIA binds to the intenal control region of JS RNA genes and promotes their transcription. Here we show that protein can be translocated from the 42S particles to 5S RNA genes, but only after treatment of the particles with ribonuclease. Nevertheless, once transferred, stable protein-DNA complexes are formed and DNase-protection experiments ahow that binding is specific to the gene promoter, covering exactly the same sequence as TFIIIA. The DNA-binding protein is identified as p48 which, after isolation by ion-exchange chromatography, wil1 bind to 5S RNA genes in the absence of ribonuclease.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
S H Kim, M K Darby, K E Joho, and D D Brown
The characterization of the TFIIIA synthesized in somatic cells of Xenopus laevis.
Genes & Dev., September 1, 1990; 4(9): 1602 - 1610.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
J Blanco, L Millstein, M A Razik, S Dilworth, C Cote, and J Gottesfeld
Two TFIIIA activities regulate expression of the Xenopus 5S RNA gene families.
Genes & Dev., October 1, 1989; 3(10): 1602 - 1612.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.