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Nucleic Acids Research, 1987, Vol. 15, No. 21 8799-8813
© 1987

Articles

Genomic organization of low copy number sequences that are associated with deca-satellite DNA in the monkey genome

Antonella Maresca1,2, Ronald E. Thayer1, Pierre Gosselin2 and Maxine F. Singer1

1Laboratory of Biochemistry, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA 2Laboratory of Molecular Endocrinology, Laval University Medical Center Quebec G1V 4G2, Canada

Received July 13, 1987. Accepted September 25, 1987.

A previously described segment of African green monkey DNA (cloned in phage {lambda}MkA) contains deca-satellite linked to DNA sequences that are estimated to occur once per genome. Sequences homologous to the low copy number sequences in {lambda}MkA are also associated with species-specific satellite DNAs in the human and mouse genomes. A second clone, {lambda}MkA, contains a monkey DNA region that is colinear and homologous to a portion of the low copy number sequences in {lambda}MkA, but no satellite sequences. The two cloned segments are markedly different starting at a point proximal to the satellite DNA region in {lambda}MkA. DNA-blotting experiments indicate that {lambda}Mk8 but not {lambda}MkA represents the typical genomic organization and that the low copy number segments occur only once per haploid genome. The data suggest that rearrangements such as deletions or inversions occurring in monkey cells account in part for the structure of {lambda}MkA. Additional rearrangements may have occurred during cloning in E. coli. This unique chromosomal region may be particularly susceptible to recombination.


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