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Nucleic Acids Research, 1987, Vol. 15, No. 21 8815-8829
© 1987

Articles

Plasmidal maintenance of composite DNA derived from polyoma related plasmid, L factor

Hiroshi Saito, Hiroshi Uehara, Tomonobu Kusano and Michio Oishi

Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Received July 8, 1987. Revised October 6, 1987. Accepted October 6, 1987.

Recently, we reported a multicopy mammalian plasmid with a structure related to polyoma (1). The plasmid, named L factor, was found at a high copy number (5,000 or pore per cell) in a subclone derived from mouse L cells. We attempted to utilize L factor as a plasmid vector for mammalian cells. A series of composite DNA consisting of L factor and a foreign (herpes simplex virus tk) were constructed. These DNA could be established as plasmids after transfection to several mouse cell lines, although the copy number of the re-established plasmids was considerably less than that observed for the original subclone. The composite DNA maintained the structure of the original DNA after prolonged culture and the copy number remained constant even with no selective pressure. A composite DNA, with no DNA sequence corresponding to polyoma T antigen, could also be established as a plasmid in a mouse L cell line in which polyoma T antigen is expressed. The potential use of the plasmid is discussed.


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