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Nucleic Acids Research, 1987, Vol. 15, No. 21 8983-8997
© 1987

Articles

Overdrive is a T-region transfer enhancer which stimulates T-strand production in Agrobacterium tumefaciens

M.J.J. van Haaren, N.J.A. Sedee, R.A. Schilperoort and P.J.J. Hooykaas

Department of Plant Molecular Biology, Biochemistry Laboratory, Leiden University Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

Received August 14, 1987. Revised October 7, 1987. Accepted October 7, 1987.

Introduction of a left or right synthetic border repeat together with the overdrive sequence in an octopine Ti-plasmid deletion mutant, lacking the right border, resulted in the complete restoration of the oncogenicity of the mutant strain. However introduction of a border repeat without the overdrive, only restored oncogenicity partially.

The overdrive sequence turned out to be able to stimulate the synthetic border mediated T-region transfer, independent of its orientation and position relative to the border repeat. Furthermore the distance between border repeat and overdrive could be enlarged, without a loss of overdrive activity. Here we enlarged the distance between the two sequences up to 6714bp. These results were confirmed by estimating the amount of single stranded T-DNA molecules from induced agrobacteria, containing the various border constructs.


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