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Nucleic Acids Research, 1987, Vol. 15, No. 22 9153-9162
© 1987

Articles

Isolation of three kinds of human endogenous retrovirus-like sequences using tRNAPro as a probe

Fumio Harada, Naoko Tsukada and Nobuyuki Kato1

Biophysics Division, Cancer Research Institute, Kanazawa University 13-1, Takaramachi, Kanazawa 920 1Virology Division, National Cancer Center Research Institute 5-1-1, Tsukiji, Chuo-ku, Tokyo 104, Japan

Received September 25, 1987. Accepted October 23, 1987.

Three kinds of human endogenous retrovirus-like sequences (HuERS-Pl, 2 and 3) were isolated from a HeLa cell genomic library using the 3'-half fragment of proline tRNA as a hybridization probe. These elements contained putative primer binding sites complementary to the 3'-terminus of proline tRNA and long terminal repeats (LTRs) characteristic of retrovirus provirus. The LTR sequence of HuERS-Pl consisted of about 690 nucleotides and contained a CAT box, a TATA box and a polyadenylation signal. A complete unit of an Alu family sequence was inserted into the 5'-LTR of one of the clones. HuERS-P2 also contained a TATA box and a polyadenylation signal in its LTR (about 840 nucleotides long), but the LTR sequence of this element was quite different from that of HuERS-Pl. Although clone HuERS-P3 contained only the 5'-LTR region, this LTR sequence contained a CAT box, a TATA box and a polyadenylation signal and was quite similar to the LTR sequence of the recently isolated human retrovirus-related sequence HuRRS-P (Kröger, B. and Horak, I. (1987) J. Virol., 61, 2071–2075). Human and simian DNAs contain 10 to 40 copies of these elements, but mouse DNA does not contain these elements.


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