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Nucleic Acids Research, 1987, Vol. 15, No. 22 9163-9176
© 1987

Articles

Sequence and centromere proximal location of a transformation enhancing fragment ansl from Aspergillus nidulans

D. Cullen*, L.J. Wilson, G.L. Grey, D.J. Henner1, G. Turner2 and D.J. Ballance2,+

Genencor Inc. South San Francisco, CA 94080, USA 1Genentech Inc. South San Francisco, CA 94080, USA 2Department of Microbiology, University of Bristol Bristol, UK

+To whom correspondence should be addressed at: Delta Biotechnology Ltd, Castle Court, Castle Boulevard, Nottingham NG7 1FD, UK

Received September 25, 1987. Revised October 16, 1987. Accepted October 27, 1987.

The Asperqillus nidulans sequence ans1, previously known to enhance transformation frequencies of pyr4-based vectors, was shown to enhance the efficiency of arqB and trpC-based vectors. Increased efficiencies could be obtained by constructing vectors containing argB and ansl or by cotransforming selectable plasmids (containing argB, trpC or pyr4) with the non-selectable ansl sequence. The preponderance of evidence suggests that the mechanism of ansl activity does not involve homologous recombination events, in spite of the presence of multiple regions of homology in the A. nidulans genome. Genetic mapping localized ansl to the vicinity of the centromere of linkage group I. The nucleotide sequence of a 1.8 Kb functional subclone of ansl was determined and found to be highly A+T rich (81%).

*Present address: USDA Forest Products Laboratory, Madison, Wl, USA


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