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Nucleic Acids Research, 1987, Vol. 15, No. 22 9263-9277
© 1987

Articles

Sequence and identification of the nucleotide binding site for the elongation factor Tu from Thermus thermophilus HB8

Lothar Seidler, Marcus Peter, Franz Meissner and Mathias Sprinzl

Laboratorium für Biochemie and Institut für makromolekulare Forschung, Universität Bayreuth Postfach 10 12 51, D-8580 Bayreuth, FRG

Received August 20, 1987. Revised October 12, 1987. Accepted October 12, 1987.

Two structural genes for the Thermus thermophilus elongation factor Tu (tuf) were identified by cross-hybridization with the tufA gene from E.coli. The sequence of one of these tuf genes, localized on a 6.6 kb Bam HI fragment, was determined and confirmed by partial protein sequencing of an authentic elongation factor Tu from T. thermophilus HB8. Expression of this tuf gene in E.coli minicells provided a low amount of immuno-precipitable thermophilic EF-Tu. Affinity labeling of the T. thermophilus EF-Tu and sequence comparison with homologous proteins from other organisms were ussd to identify the gusnosine-nucleotide binding domain.


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