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Nucleic Acids Research, 1987, Vol. 15, No. 22 9451-9459
© 1987

Articles

The primary structure of rat ribosomal protein S8

Yuen-Ling Chan, Alan Lin, Veronica Paz and Ira G. Wool*

Department of Biochemistry and Molecular Biology, University of Chicago Chicago, IL 60637, USA

*To whom correspondence should be addressed

Received September 21, 1987. Accepted October 12, 1987.

The amino acid sequence of rat ribosomal protein S8 was deduced from the sequence of nucleotides in a recombinant cDNA and confirmed from the NH2- and carboxyl-terminal amino acid sequences of the protein. Ribosomal protein S8 contains 207 amino acids (the NH2-terminal methionine is removed after translation of the mRNA) and has a molecular weight of 23,928. Hybridization of the cDNA to digests of nuclear DNA suggests that there are 7–9 copies of the S8 gene. Ribosomal protein S8 contains a possible internal repeat that has 12 or 13 residues, is basic, and occurs 5 times in the protein.


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