Skip Navigation

This Article
Right arrow Print PDF (723K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (41)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Mitchelson, K.
Right arrow Articles by Moss, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mitchelson, K.
Right arrow Articles by Moss, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 22 9577-9596
© 1987

Articles

The enhancement of ribosomal transcription by the recycling of RNA polymerase I

Keith Mitchelson1 and Tom Moss2,*

Biophysics Laboratories, Portsmouth Polytechnic St Michael's Building, White Swan Road, Portsmouth PO1 2DT, UK

*To whom correspondence should be addressed

Received July 27, 1987. Accepted October 9, 1987.

It has been suggested that the tandemly repeated ribosomal genes of eukaryotes may be subject to a special mechanism of transcriptional enhancement, called Readthrough Enhancment, in which transcription factors are recycled. Recent experiments with the mouse ribosomal. genes, although consistent with this possibility, were unable to distinguish between true Readthrough Enhancement and promoter occlusion. To test directly for Readthrough Enhancement, the pre-ribosomal RNA of Xenopus laevis was prematurely terminated within the 18S gene on a circular template. This premature termination was found to reduce the efficiency of pre-ribosomal RNA promotion in cis by 80 to 90%. Since the pre-ribosomal RNA is normally terminated only 213 base pairs upstream of its own initiation site, the results strongly suggest that the recycling of RNA polymerase, or Readthrough Enhancement, is a means by which ribosomal transcription is enhanced in Xenopus laevis.

1Present address: Glasshouse Crops Research Institute, Worthing Road , Littlehampton, West Sussex BN17 6LP, UK

2Present address: Centre de Recherche sur le Cancer de I'Universite Laval, L'Hotel Dieu de Quebec, 11 Cote du Palais, Quebec G1R 216, Canada


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. Roux-Rouquie and M. Marilley
Modeling of DNA local parameters predicts encrypted architectural motifs in Xenopus laevis ribosomal gene promoter
Nucleic Acids Res., September 15, 2000; 28(18): 3433 - 3441.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
V Sirri, P Roussel, and D Hernandez-Verdun
The mitotically phosphorylated form of the transcription termination factor TTF-1 is associated with the repressed rDNA transcription machinery
J. Cell Sci., January 10, 1999; 112(19): 3259 - 3268.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Kang, T. J. Yokoi, and M. J. Holland
Binding Sites for Abundant Nuclear Factors Modulate RNA Polymerase I-dependent Enhancer Function in Saccharomyces cerevisiae
J. Biol. Chem., December 1, 1995; 270(48): 28723 - 28732.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
B McStay and R H Reeder
An RNA polymerase I termination site can stimulate the adjacent ribosomal gene promoter by two distinct mechanisms in Xenopus laevis.
Genes & Dev., July 1, 1990; 4(7): 1240 - 1251.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
S L Henderson, K Ryan, and B Sollner-Webb
The promoter-proximal rDNA terminator augments initiation by preventing disruption of the stable transcription complex caused by polymerase read-in.
Genes & Dev., February 1, 1989; 3(2): 212 - 223.
[Abstract] [PDF]

Home page
 ScienceHome page
I. Dawid and T. Sargent
Xenopus laevis in developmental and molecular biology
Science, June 10, 1988; 240(4858): 1443 - 1448.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.