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Nucleic Acids Research, 1987, Vol. 15, No. 24 10211-10232
© 1987

Articles

Characterization of human DNA sequences synthesized at the onset of S-phase

Carla Tribioli, Giuseppe Biamonti, Mauro Giacca, Marco Colonna, Silvano Riva and Arturo Falaschi*

Istituto di Genetica Biochimica ed Evoluzionistica del CNR Via Abbiategrasso, 207, 1-27100 Pavia, Italy

Received September 25, 1987. Revised November 25, 1987. Accepted November 25, 1987.

We developed a method of enrichment for DNA replicated at the onset of S-phase in synchronized human HL60 cells. About 200 such sequences were cloned. The analysis of this selected DNA sample showed that: 1) the cloned DNA fragments derive from a limited number (750–1500) of replicons; 2) there is no extensive homology between different DNA fragments; 3) they are not significantly enriched in highly repeated sequences; 4) they are enriched in snap-back (Cot = o) DNA. The sequence of the longest fragment revealed the presence of numerous signals collected in a few hundred nucleotides: 1) homology with the origin of replication of human Papovaviruses usually associated with potential stem-loop structures; 2) binding sites for known transcription factors and for another nuclear factor; 3) potential binding sites for the chromosome "scaffold".

*Present address: International Centre for Genetic Engineering and Biotechnology, Padriciano 99, 1-34012 Trieste, Italy


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