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Nucleic Acids Research, 1987, Vol. 15, No. 24 10437-10453
© 1987

Articles

A composite transposon 3' to the cow fetal globin gene binds a sequence specific factor

Carolyn R. Zelnick, Douglas J. Burks1 and Craig H. Duncan*

Division of Basic Science, IDR 727, Children's Hospital Research Foundation, Elland and Bethesda Aves Cincinnati, OH 45229, USA 1Department of Biology, Wilmington College Wilmington, OH 45177, USA

*To whom correspondence should be addressed

Received August 4, 1987. Revised November 2, 1987. Accepted November 10, 1987.

Two unusual sequence organizations were found within the ß-globin locus of the cow. Each was a composite, consisting of closely linked Alu-type repeats with a short stretch of genomic non-repetitive sequence, called a lagan, sandwiched between. One lagan was found 3' to the fetal globin gene, while the second lay between the adult globin gene and a globin pseudogene. Southern blot analysis indicated that both lagans appeared twice within the cow haploid genome, with the second copies lying outside the cow ß-globin locus. One of these non-globin locus homologues was cloned and subjected to sequence analysis. Comparison of the DNA sequence data showed that the lagan-Alu composite was transposed as a unit. The lagan 3' to the cow fetal globin gene contains the recognition site for a sequence specific DNA binding factor. This factor was present in extracts from fetal, but not from adult cow tissues.


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