Nucleic Acids Research, 1987, Vol. 15, No. 3 987-1003
© 1987
Articles |
Structural analysis of the Drosophila rpA1 gene, a member of the eucaryotic ‘A’ type ribosomal protein family
Department of Developmental Genetics and Anatomy, School of Medicine, Case Western Reserve University 2119 Abington Road, Cleveland, OH 44106, USA
*To whom correspondence should be addressed
Received October 7, 1986. Revised January 9, 1987. Accepted January 9, 1987.
The expression of ribosomal protein (r-protein) genes is uniquely regulated at the translational level during early development of Drosophila. Here we report results of a detailed analysis of the r-protein rpAl gene. A cloned DNA sequence coding for rpAl has been identified by hybrid-selected translation and amino acid composition analysis. The rpAl gene was localized to polytene chromosome band 53CD. The nucleotide sequence of the rpAl gene and its cDNA have been determined. rpAl is a single copy gene and sequence comparison between the gene and its cDNA indicates that this r-protein gene is intronless. Allelic restriction site polymorphisms outsite of the gene were observed, while the coding sequence is well conserved between two Drosophila strains. The protein has unusual domains rich in Ala and charged residues. The rpAl is homologous to the "A" family of eucaryotic acidic r-proteins which are known to play a key role in the initiation and elongation steps of protein synthesis.
1Present address: Department of Biochemistry, Tianjing Medical College, Tianjing, People's Republic of China
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Shimizu, M. Nakagaki, Y. Nishi, Y. Kobayashi, A. Hachimori, and T. Uchiumi Interaction among silkworm ribosomal proteins P1, P2 and P0 required for functional protein binding to the GTPase-associated domain of 28S rRNA Nucleic Acids Res., June 15, 2002; 30(12): 2620 - 2627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Surdej and M. Jacobs-Lorena Developmental Regulation of bicoid mRNA Stability Is Mediated by the First 43 Nucleotides of the 3' Untranslated Region Mol. Cell. Biol., May 1, 1998; 18(5): 2892 - 2900. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. Szick, M. Springer, and J. Bailey-Serres Evolutionary analyses of the 12-kDa acidic ribosomal P-proteins reveal a distinct protein of higher plant ribosomes PNAS, March 3, 1998; 95(5): 2378 - 2383. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-i. Ito and M. Jacobs-Lorena Functional Mapping of Destabilizing Elements in the Protein-coding Region of the Drosophila fushi tarazu mRNA J. Biol. Chem., June 22, 2001; 276(26): 23525 - 23530. [Abstract] [Full Text] [PDF]
|
|