Nucleic Acids Research, 1987, Vol. 15, No. 4 1477-1492
© 1987
Articles |
Sequence and regulatory responses of a ribosomal protein gene from the fission yeast Schizosaccharomyces pombe
Institut für Biochemie and Molekularbiologie, Freie Universität Berlin Ehrenbergstr. 26–28, D-1000 Berlin 33, FRG
Received November 24, 1986. Revised January 16, 1987. Accepted January 16, 1987.
We have determined the nucleotide sequence and mapped the 5' and 3' termini of a ribosomal protein gene. The gene is transscribed into a RNA molecule of about 770 nt and appears to initiate at multiple sites, as judged by SI nuclease analysis. Gene dosage experiments with a plasmid born gene leads to a proportional increase of the messenger RNA, but not to an over-production of the protein, suggesting a posttranscriptional control mechanism. However, the heat shock response of this gene indicates that there is also a potential for transcriptional control. Comparison of the 5' flanking region of this gene with the ribosomal protein gene S 6 from Schizosaccharomyces pombe and with ribosomal protein genes from Saccharomyces cerevisiae revealed homologous sequences, which may be involved in the regulation of ribosomal protein genes.
+Present address: Deutsches Krebsforschungs Zentrum, Institut für Experimentelle Pathologie, Im Neuenheimer Feld 280, D-6900 Heidelberg, FRG