Nucleic Acids Research, 1987, Vol. 15, No. 4 1615-1625
© 1987
Articles |
Characterization of the 5'-flanking region for the human fibrinogen ß gene
INSERM U217, DRF/Laboratoire d'Hématologie C.E.N. G., 85X, F 38041, Grenoble Cedex, France
Received October 31, 1986. Revised January 21, 1987. Accepted January 28, 1987.
To identify the possible regulatory sequences in the genetic expression of fibrinogen, a human genomic DNA library raised in 
EHBL 4 phage was screened using cDNA probes coding for the A
, Bß and 
chains of human fibrinogen. The entire fibrinogen locus was characterized and its organization analysed by means of hybridization and restriction mapping. Among the clones identified, a single recombinant phage contained the gene and its 5'-and 3'-flanking regions. A 1.5 kb fragment of the immediate 5'-flanking region was sequenced and S1 mapping experiments revealed three transcription start points. Comparison of this sequence with that previously reported for the same region upstream from the human gene revealed no significant homology which suggests that the potential promoting sequences of these genes are different. In contrast, comparison of the 5'-flanking regions of human and rat ß genes revealed a 142 bp sequence of 80% homology situated 16 bp upstream from the human gene. This highly conserved region may well represents a potential candidate for a regulatory sequence of the human ß gene.
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