Nucleic Acids Research, 1987, Vol. 15, No. 4 1643-1659
© 1987
Articles |
Posttranscriptional regulation of c-fos mRNA expression
Kernforschungszentrum Karlsruhe, Institut für Genetik und Toxikologie, and Universität Karlsruhe, Institut für Genetik Postfach 3640, D-7500 Karlsruhe 1 *European Molecular Biology Laboratory Postfach 102209, D-6900 Heidelberg, FRG
Received September 25, 1986. Revised December 17, 1986. Accepted January 19, 1987.
The transient induction of c-fos mRNA and protein suggests that regulation occurs not only by transcriptional activation but also at the level of turnover of the gene product. Here we present evidence for the rapid turnover of c-fos aRNA and some of the requirements for its specific degradation. The half life of induced mature cytoplasmic c-fos mRNA is 9 min in both serum-starved and growing primary human fibroblasts and in NIH 3T3 cells. A structure present at the 3' end of the c-fos mRNA molecule is involved in its low stability since the substitution or the removal of the untranslated 3' portion prolongues the RNA life time. The rapid turnover of fos mRNA requires, in addition, continued protein synthesis. Treatment of cells with cycloheximide stabilizes c-fog mRNA. Washing out cycloheximide reestabilishes the rapid turnover. Both changes occur with lag periods of less than 17 minutes.
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