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Nucleic Acids Research, 1987, Vol. 15, No. 4 1745-1752
© 1987

Articles

Catalytic activity of the nucleic acid component of the 1,4-{alpha}-glucan branching enzyme from rabbit muscles

T.A. Shvedova, G.A. Korneeva, V.A. Otroshchenko and T.V. Venkstern*

A.N.Bach Institute of Biochemistry USSR Academy of Sciences, Moscow, USSR *Institute of Molecular Biology USSR Academy of Sciences, Moscow, USSR

Received December 19, 1986. Accepted January 15, 1986.

2.5 S RNA, the nucleic acid component of the 1,4-{alpha}-D-glucan: 1,4-{alpha}6-D-glucan 6-{alpha}-(1,4-{alpha}-glucano)-transferase from rabbit muscles, devoid of any protein, catalyses the branching reaction, as does the holoenzyme. The conclusion is drown that 2.5 S RNA is a ribozyme. To get an insight into the significance of different parts of the molecule for the catalytic activity of 2.5 S RNA, a large fragnent isolated from its partial RNAase A digest was investigated. This fragment which proved to be the middle part of polyribonucleotide chain containing all modified nucleotides exerts some catalytic activity, too.


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