Nucleic Acids Research, 1987, Vol. 15, No. 7 2959-2969
© 1987
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Molecular cloning and sequence analysis of a mouse Y chromosome RNA transcript expressed in the test is
Unité d'Immunogénétique Humaine, INSERM U.276, Institut Pasteur 75724 Paris, Cedex 15, France
Received December 29, 1986. Revised February 27, 1987. Accepted March 17, 1987.
Using a Y specific probe (pY353/B) taken from a flow sorted mouse Y chromosome library we have identified a family of RNA transcripts encoded by the Y chromosome. These transcripts which are approximately 1.3 Kb in length are present in testis PolyA+ RNA but can not be detected in either male liver, spleen, kidney, brain, heart or lung tissues. Isolation and sequence analysis of a corresponding cDNA shows it to contain a potential coding sequence of 696bp. These data show that the Y chromosome is transcriptionally active in the adult mouse testis.
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