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Nucleic Acids Research, 1987, Vol. 15, No. 8 3199-3220
© 1987

Articles

Cross index for improving cloning selectivity by partially filling in 5'-extensions of DNA produced by type II restriction endonucleases

Christopher Korch

Department of Microbiology, University of Umea S-901 87 Umea, Sweden

Received January 20, 1987. Revised April 3, 1987. Accepted April 3, 1987.

A cross index is presented for using the improved selectivity offered by the Rung and Wensink (Nuc1. Acids Res. 12, 1863–1874, 1984) method of partially filling in 5'-extensions produced by type II restriction endonucleases. After this treatment, DNA fragments which normally cannot be ligated to one another, can be Joined providing that complementary cohesive ends have been generated. The uses of this technique, which include the prevention of DNA fragments (both vector and insert) auto-annesling, are discussed.


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