Nucleic Acids Research, 1987, Vol. 15, No. 8 3241-3255
© 1987
Articles |
RNA-protein cross-linking in Escherichia coli 30S ribosomal subunits; determination of sites on 16S RNA that are cross-linked to proteins S3, S4, S7, S9, S10, S11, S17, S18 and S21 by treatment with bis-(2-chloroethyl)-methylamine
ffler1
Max-Planck-Institut f
r Molekulare Genetik, Abteilung Wittmann D-1000 Berlin-Dahlem, FRG
1Institut für Mikrobiologie, Medizinische Fakult
t, Univcrsitt Innsbruck A-6020 Innsbruck, Austria
*To Whom correspondence should be addressed
RNA-protein cross-links were introduced into E. coli 30S ribosomal subunits by treatment with bis-(2-chloroethyl)-methyl-amine. After partial nuclease digestion of the RNA moiety, a number of cross-linked RNA-protein complexes were isolated by a new three-step procedure. Protein and RNA analysis of the individual complexes gave the following results: proteins S4 and S9 are cross-linked to the 16S RNA at positions 413 and 954, respectively. Proteins SII and S21 are both cross-linked to the RNA within an oligonucleotide encompassing positions 693–697, and proteins S17, S10, S3 and S7 are cross-linked within oligonucleo-tides encompassing positions 278–280, 1139–1144, 1155–1158, and 1531–1542, respectively. A cross-link to protein S18 was found by a process of elimination to lie between positions 845 and 851.
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