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Nucleic Acids Research, 1987, Vol. 15, No. 8 3369-3383
© 1987

Articles

Sequence and S1 nuclease mapping of the 5' region of the dihydrofolate reductase—thymidylate synthase gene of Leishmania major

Geoffrey M. Kapler1,2, Kang Zhang1 and Stephen M. Beverley1,*

1Department of Pharmacology, Harvard Medical School Boston, MA 02115, USA 2Department of Genetics, Harvard Medical School Boston, MA 02115, USA

*To whom correspondence should be addressed

Received October 28, 1986. Revised March 12, 1987. Accepted March 12, 1987.

The 5' structure of mRNA transcribed from the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene of the protozoan parasite Leishmania major has been characterized. S1 nuclease mapping identifies a heterogenous 5' structure which is not affected by growth phase or developmental stage. The DNA sequence of the 5' region of the DHFR-TS gene does not reveal homology with other trypanosomatid genes, eukaryotic consensus genetic elements, or the mammalian DHFR promoter element. This latter finding is especially significant as we show that the 5' region of the E. coli DHFR gene exhibits homology to the mammalian DHFR promoter element, despite their greater evolutionary distance.


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