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Nucleic Acids Research, 1987, Vol. 15, No. 8 3479-3491
© 1987

Articles

Organ-specific and dosage-dependent expression of a leaf/stem specific gene from potato after tagging and transfer into potato and tobacco plants

Jorg Stockhaus*, Peter Eckes+, Astrid Blau, Jeff Schell and Lothar Willmitzer*

Max-Planck-Institut für Züchtungsforschung 5000 Köln 30, FRG

Received February 6, 1987. Revised April 3, 1987. Accepted April 3, 1987.

ST-LS1, a single copy gene from potato displaying a leaf/stem specific gene expression, was tagged by an exon modification and introduced into both potato and tobacco cells using Agrobacteri-um vectors. After regeneration of whole plants, the expression of the tagged gene was analyzed with respect to its organ specifity and compared to the expression of the corresponding resident gene. The expression of the transferred gene in transgenic plants closely followed the expression of the resident gene. No marked influence of the plant species serving as host was observed. The level of expression of the introduced gene varied by a factor of at least 100 in independent transformants when normalized to the expression of the resident gene. Southern analysis performed on the transformed plants indicated a correlation between copy number of the introduced gene and its expression level. The activity of the tagged gene as well as of the resident gene was significantly inhibited by treatment of the transgenic plants with the herbicide norfluorazon, indicating that this gene activity is dependent on the presence of functional chloroplasts in the leaves.

*Present address: IGF Berlin, Ihnestrasse 63, 1000 Berlin 33, FRG

+Present address: Hoechst AG, Frankfurt/Main, FRG


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