Skip Navigation

This Article
Right arrow Print PDF (1190K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bower, M.
Right arrow Articles by Wilson, W. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bower, M.
Right arrow Articles by Wilson, W. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1987, Vol. 15, No. 8 3531-3547
© 1987

Articles

Synthesis and characterization of oligodeoxyribonucleotides containing terminal phosphates. NMR, UV spectroscopic and thermodynamic analysis of duplex formation of [d(pGGAATTCC)]2, [d(GGAATTCCp)]2 and [d(pGGAATTCCp)]2

Margarete Bower1, Michael F. Summers1, Barbara Kell3, Joel Hoskins2,* and W. David Wilson3

1Biophysics Laboratory, Division of Biochemistry and Biophysics, Food and Drug Administration 8800 Rockville Pike, Bethesda, MD 20892 2Molecular Pharmacology Laboratory, Division of Biochemistry and Biophysics, Food and Drug Administration 8800 Rockville Pike, Bethesda, MD 20892 3Department of Chemistry, Georgia State University Atlanta, GA 30303-3083, USA

Received January 14, 1987. Revised March 19, 1987. Accepted April 3, 1987.

Derivatives of the oligomer [d(GGAATTCC)]2 with 5' (5'-P), 3' (3'-P) and both 5' and 3' (5', 3'-P2) terminal phosphate groups have been synthesized and studied by temperature dependent UV and NMR spectroscopic methods. Thermo-dynamic studies of the helix to strand transition indicate that addition of 3' phosphate groups has very little effect on the {Delta}G° for helix formation at 37°C while addition of 5' phosphate groups adds approximately -0.5 kcal/mole to the {Delta}G° for duplex formation. The helix stabilization by 5' phosphate groups occurs at salt concentrations of 0.1 M and above, and is primarily enthalpic in origin. Tm studies as a function of ionic strength also indicate that the oligomers fall into two groups with the parent and 3'-P derivatives being similar but less stable than the 5'-P and 5', 3'-P2 derivatives. Imino proton and 31P NMR studies also divide the oligomers into these same two groups based on spectral comparisons and temperature induced chemical shift and linewidth changes. 31P NMR analysis suggests that addition of 5' phosphate groups results in a small change in phosphodiester torsional angles in the g, t to g, g direction, indicating improved base stacking at the 5' end of the modified oligomer. No such changes are seen at the 3' end of the oligomer on adding 3' phosphate groups.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Biophys. JHome page
Z.-J. Tan and S.-J. Chen
Nucleic Acid Helix Stability: Effects of Salt Concentration, Cation Valence and Size, and Chain Length
Biophys. J., February 15, 2006; 90(4): 1175 - 1190.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.