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Nucleic Acids Research, 1987, Vol. 15, No. 9 3689-3702
© 1987

Articles

Bacillus subtilis phage SPR codes for a DNA methyttransferase with triple sequence specificity

Ursula Gunthert* and Luzia Reiners

Max Planck Institut für molelculare Genetik D-1000 Berlin 33, FRG

Received February 9, 1987. Revised April 10, 1987. Accepted April 10, 1987.

SPR, a temperate Bacillus subtilis phage, codes for a DNA methyltrans-ferase that can methylate the sequences GGCC (or GGCC) and CCGG at the cytosines indicated. We show here that it can also methylate the sequence CC(A/T)GG and protect it from cleavage with EcoRII and Apyl. This methylation can be seen in vivo as well as in vitro with purified SPR methyltransferase. SPR19 and SPR83 are two mutant phages, defective in GGCC or CCGG methylation, respectively. These mutants have not lost their ability to methylate CC(AA)GG sites. Mutation SPR26 has lost the ability to methylate all three sites. Thus the SPR methyltransferase codes for three genetically distinguishable methylation abilities.

*Present address: Kernforschungszentrum Karlsrube, Institut für Genetick and Toxikologie, Postfach 3640, D-7500 Karlsrube 1, FRG


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