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Nucleic Acids Research, 1987, Vol. 15, No. 9 3761-3772
© 1987

Articles

A new solid-phase synthesis of oligoribonudeotides by the pbosphoro-p-anisidate method using tetrahydrofuranyl protection of 2'-hydroxyl groups

Shigenori Jwai, Emi Yamada, Miyuki Asaka, Yoji Hayase, Hideo Inoue and Eiko Ohtsuka

Faculty of Pharmaceutical Sciences, Hokkaido University Sapporo 060, Japan

Received March 17, 1987. Accepted April 6, 1987.

Six nonaribonucleotides containing the 5'-splice site, one complementary nonamer and an octadecamer containing the 3'-splice site have been synthesized on a polymer support using the phosphoro-p-anisidate method. A 5'-linked 2'-O-tetra-hydrofuranyl-N-protected nucleoside 3'-(o-chlorophenyl)phos-phoro-p-anisidate was used as the starting nucleotide, and the chain elongated in the 3'-direction by removing the p-anisidate protecting group with isoamyl nitrite under neutral conditions. The octadecamer has been synthesized using dinucleotide blocks and a 3'-terminal trinucleotide.


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