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Nucleic Acids Research, 1987, Vol. 15, No. 9 3891-3906
© 1987

Articles

Structure and sequence of a UDP glucose pyrophosphorylase gene of Dictyostelium discoideum

Jack A. Ragheb and Robert P. Dottin*

Department of Biology, The Johns Hopkins University Baltimore, MD 21218, USA

*To whom reprint requests should be sent

Received December 3, 1986. Revised April 10, 1987. Accepted April 10, 1987.

Cell-cell contact and exogenous cAMP regulate the expression of uridine diphosphoglucose pyrophosphorylase (UDPGP) of Dictyostelium diacoideum (B. Haribabu, A. Rajkovic and R. P. Dottin, 1986, Dev. Biol., Vol. 113, 436–442). cAMP appears to regulate gene expression in Dictyostelium by transmembrane signal transduction (B. Haribabu and R. Dottin, 1986, Hol. Cell. Biol. 62402–2408). To further characterize the mechanism of action of cAMP on the expression of this gene and the nature of the defects in UDPGP mutants that abort development, we sequenced the cDNA and the genomic DNA, including intervening and flanking sequences. The deduced amino acid sequence predicts a polypeptide of 57, 893 d. molecular weight. Three short (100–200 nucleo-tides) A+T rich introns occur within the coding sequences but only one of them contains a sequence TAACTAAC, similar to the yeast lariat acceptor site. The 5' flanking sequences are also A+T rich and contain an oligo A tract (–14 to –24), a TATA box (–25 to –32), and a short G+C rich region (–63 to –101) which may be a control region. From –196 to –209 is a sequenc AAAGTAGTATTCAA which matches in 11 of its 14 nucleotides, a sequence found upstream from the hormonally regulated P-enolpyruvate carboxykinase gene of rat.


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