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Nucleic Acids Research, 1988, Vol. 16, No. 1 135-150
© 1988

Articles

An archaebacterial RNA polymerase binding site and transcription initiation of the hisA gene in Methanococcus vannielii

James W. Brown1, Michael Thomm2, Gregory S. Beckler1, Gerhard Frey2, Karl O. Stetter2 and John N. Reeve1,3

1Molecular, Cellular and Developmental Biology Program, Ohio State University Columbus, OH 43210, USA 2Lehrstuhl für Mikrobiologie, Universität Regensburg D-8400 Regensburg, FRG 3Department of Microbiology, Ohio State University Columbus, OH 43210, USA

Received September 23, 1987. Revised December 3, 1987. Accepted December 3, 1987.

Transcription initiation of the hisA sent in vico. in the archaebacterium Methanococcus vannielii, as determined by nuclease S1 and primer extension analyses, occurs 73 base pairs (bp) upstream of the translation initiation site. Binding of M. vannielii RHA polymerase protects 43 bp of DNA. from 35 bp upstream (–35) to 8 bp downstream (+8) of the hisA mRNA initiation site, from digestion by DNase I and exonuclease III. An A+T rich region, with a sequence which conforms to the consensus sequence for promoters of stable RNA-encoding genes in methanogens, is found at the same location (–25) upstream of the polypeptide-encoding hisA gene. It appears therefore that a TATA-like sequence is also an element of promoters which direct transcription of polypeptide-encoding genes in this archaebacterium.


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